Diffuse reflectance spectroscopy of human liver tumor specimens – towards a tissue differentiating optical biopsy needle using light emitting diodes.


Keller A1, Bialecki P1, Wilhelm TJ2,3, Vetter MK1,3.

Biomed Opt Express. 2018 Feb 8;9(3):1069-1081


Significant numbers of liver biopsies fail to yield representative tissue samples. This study was conducted to evaluate the ability of LED-based diffuse reflectance spectroscopy to discriminate tumors from liver parenchyma. Ex vivo spectra were acquired from malignant lesions and liver parenchyma of 32 patients who underwent liver resection using a white light source and several LEDs. Integrated spectra of two combined LEDs with emission peaks at 470 nm and 515 nm were classified with 98.4% sensitivity and 99.2% specificity. The promising results could yield to a simple handheld and cost-efficient tool for real-time tissue differentiation implemented in a biopsy needle.

PMCID: PMC5846514 DOI: 10.1364/BOE.9.001069